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Saturday, May 9, 2020 | History

2 edition of Expression of rotavirus antigens in adenovirus based vectors found in the catalog.

Expression of rotavirus antigens in adenovirus based vectors

Chang-Chih Liu

Expression of rotavirus antigens in adenovirus based vectors

by Chang-Chih Liu

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  • 19 Currently reading

Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (M.Sc.) - University of Warwick, 1996.

StatementChang-Chih Liu.
The Physical Object
Pagination173p.
Number of Pages173
ID Numbers
Open LibraryOL17496012M

  Adenovirus 5 (Ad5) causes mild respiratory tract infections in humans but its main claim to fame is as a potential vaccine vector for key diseases such as HIV/AIDS and malaria. Its potential Cited by: Gene therapy with viral vectors has significantly advanced in the past few decades, with adenovirus being one of the most commonly employed vectors for cancer gene therapy. Adenovirus vectors can be divided into 2 groups: (1) replication-deficient viruses; and (2) replication-competent, oncolytic (OVs) viruses. Replication-deficient adenoviruses have been explored as vaccine carriers and gene.

  ABSTRACT Adenovirus is a common infectious pathogen in both children and adults. It is a significant cause of morbidity in immunocompetent people living in crowded living conditions and of mortality in immunocompromised hosts. It has more recently become a popular vehicle for gene therapy applications. The host response to wild-type infection and gene therapy vector exposure involves both Cited by: Many of the adenovirus serotypes can multiply in the small intestine, but only types 40 and 41 have been strongly associated with gastroenteritis (Grimwood et al. ). Adenovirus is considered to be second only to rotavirus in terms of its significance as a cause of childhood gastroenteritis (Crabtree et al. ). Studies covering the.

Rotavirus antigen has also been identified in the serum of patients 3–7 days after disease onset, but at present, routine diagnostic testing is based primarily on testing of fecal specimens. Epidemiology Occurrence Rotavirus occurs throughout the world. The incidence of rotavirus is similar in developed and developing countries. Components of the ViraPower™ Adenoviral Expression System The ViraPower™ Adenoviral Expression System facilitates highly efficient, in vitro or in vivo delivery of a target gene to dividing and non-dividing mammalian cells using a replication-incompetent adenovirus. Based on the secondgeneration vectors developed by Bett et al., , the ViraPower™ Adenoviral Expression .


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Expression of rotavirus antigens in adenovirus based vectors by Chang-Chih Liu Download PDF EPUB FB2

An infectious recombinant human adenovirus type 5 (Ad5) vector, AdG12, which carries the glycoprotein gene of vesicular stomatitis virus (VSV) and expresses that gene in cultured HeLa cells was used to examine the host range of insert expression by human Ad by: Adenoviral Vectors for Gene Therapy, Second Edition provides detailed, comprehensive coverage of the gene delivery vehicles that are based on the adenovirus that is emerging as an important tool in gene therapy.

These exciting new therapeutic agents have great potential for the treatment of disease, making gene therapy a fast-growing field for. Expression of the OSU rotavirus outer capsid protein VP4 by an adenovirus recombinant. M Gorzigliaand A Z Kapikian. Epidemiology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland Author information Copyright and License information.

Copyright by: Animal Models of Gene Therapy for Cardiovascular Disease. Introduction; 2. Adenoviral Vectors for Cardiovascular Gene Therapy; 3. Animal Models for Cardiovascular Gene Transfer; 4. Conclusions; Polymer-Anchored Adenovirus as a Therapeutic Agent for Cancer Gene Therapy.

Introduction; 2. Polymer Coating on Adenovirus Surface; 3. They are attractive vaccine vectors as they induce both innate and adaptive immune responses in mammalian hosts. Currently, adenovirus vectors are being tested as subunit vaccine systems for numerous infectious agents ranging from malaria to HIV Additionally, they are being explored as vaccines against a multitude of tumor-associated by:   Anwar M Hashem, Abdullah Algaissi, Anurodh Shankar Agrawal, Sawsan S Al-amri, Rowa Y Alhabbab, Sayed S Sohrab, Abdulrahman S.

Almasoud, Naif Khalaf Alharbi, Bi-Hung Peng, Marsha Russell, Xuguang Li, Chien-Te K Tseng, A Highly Immunogenic, Protective, and Safe Adenovirus-Based Vaccine Expressing Middle East Respiratory Syndrome Coronavirus S1-CD40L Fusion Protein Cited by: 5.

The data suggest that for an experimental adenovirus-based HIV-1 vaccine, transgene expression in non-permissive cells in vitro can be reproducibly modulated by the presence of additional replication-incompetent adenovirus.

This modulation can occur in circumstances where another transgene-encoding adenovirus is present, as well as with the addition of adenovirus that does not contain the Cited by: 4. Novel adenovirus vaccine vectors based on the enteric-tropic serotype 41 Article (PDF Available) in Vaccine 25(11) April with 61 Reads How we measure 'reads'.

Systemic administration of adenoviral vectors leads to activation of innate and antigen-specific immunity. In an attempt to diminish T and B cell-specific immune responses to E1-deleted adenoviral vectors, capsid proteins were modified with various activated Cited by: Recombinant adenoviruses have become a popular method for protein expression because the rate of infection and the subsequent yield of recombinant protein are typically quite high.

Adenoviruses are especially useful in infecting cell lines that have low transfection efficiency with liposome. We employ innovative technologies to make your adenoviral studies simpler and more successful.

Although high-level transient transgene expression afforded by first-generation Ad vectors may be adequate, or even desirable, for many gene transfer and gene therapy applications, the transient nature of expression kinetics renders these vectors unsuitable when prolonged, stable expression is by:   In the past decade, adenovirus vectors have generated tremendous interest, especially in gene therapy applications.

In the so-called ‘first generation’ adenovirus vectors, the transgenes are Cited by:   Based on two new adenovirus expression cassettes, we have constructed a series of Ad transfer vectors for the overexpression of one or two genes either in a dicistronic configuration or with separate expression cassettes.

Inclusion of the green or blue. Adenovirus (AdV) vectors are viruses that have been engineered to carry exogenous DNA into host cells. The genetic modifications include removal of essential genes which renders the vector replication defective. AdV serotypes 2 and 5 are the most commonly used as vectors in vaccine development.

Abstract. Based on two new adenovirus expression cassettes, we have constructed a series of Ad transfer vectors for the over-expression of one or two genes either in a dicistronic configuration or with separate expression by: This together with the finding that genomic rearrangements in AdE1-vectors containing inverted repeats strictly depended on viral DNA replication was the basis for the development of an adenovirus-based expression system that is exclusively activated in hepatic metastases after systemic vector administration.

In these vectors, a transgene is linked to a highly tumor-specific promoter during viral. @article{osti_, title = {Development of an adenoviral vector with robust expression driven by p53}, author = {Bajgelman, Marcio C and Biotechnology Program, Biomedical Sciences Institute, University of Sao Paulo and Millennium Institute-Gene Therapy Network, Ministry of Science and Technology and Strauss, Bryan E and Biotechnology Program, Biomedical Sciences Institute.

Adenovirus Vectors. Adenovirus vectors are probably the most frequently used viral vectors, mainly because of the high levels of transgene expression that can be obtained in a broad range of host cells.

While the early versions of adenovirus vectors caused toxic effects and typically induced strong immune responses, more recent versions (carrying deletions in nonessential viral genes) exhibit reduced toxicity and improved gene expression.

Abstract Here we describe a series of replication-defective adenovirus vectors designed to express transgene products from two expression cassettes placed into the deleted E1 and E3 domains.

Vectors that contained an E1 cassette with a cytomegalovirus promoter in the forward orientation and an E3 cassette with the chicken β-actin promoter in the reverse orientation grew to acceptable yields Cited by: 9.

Due to the very efficient nuclear entry mechanism of adenovirus and its low pathogenicity for humans, adenovirus‐based vectors have become gene delivery vehicles that are widely used for transduction of different cell types, especially for quiescent, differentiated cells, in basic research, in gene therapy applications, and in vaccine Cited by:.

Pink Book’s Chapter on Rotavirus pdf icon [12 pages] Epidemiology & Prevention of Vaccine-Preventable Diseases textbook.

Publications MMWR. Hallowell BD, Parashar UD, Curns A, DeGroote NP, Tate JE. Trends in the Laboratory Detection of Rotavirus Before and After Implementation of Routine Rotavirus Vaccination — United States, –Adenovirus vectors are the most commonly employed vector for cancer gene therapy.

They are also used for gene therapy and as vaccines to express foreign antigens. Adenovirus vectors can be replication-defective; certain essential viral genes are deleted and replaced by a cassette that expresses a foreign therapeutic by:   Rotavirus antigens were found in samples (%), adenovirus antigens in samples (%), and rotavirus and adenovirus antigens together in 16 samples (%).

There was a seasonal change in rotavirus gastroenteritis (P of rotavirus cases in those with diarrhea by 0 Cited by: 5.